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  1. Soil lead (Pb) contamination is a major environmental and public health risk. Switchgrass (Panicum virgatum), a second-generation biofuel crop, is potentially useful for the long-term phytoremediation and phytoextraction of Pb contaminated soils. We evaluated the efficacy of a coordinated foliar application of plant growth regulators and soil fungicide and a chelator in order to optimize phytoextraction. Plants were grown in soil culture under controlled conditions. First, three exogenous nitric oxide (NO) donors were evaluated at multiple concentrations: (1) S-nitroso-N-acetylpenicillamine (SNAP); (2) sodium nitroprusside (SNP); and (3) S-nitrosoglutathione (GSNO). Second, the effect of SNP (0.5 μM) was examined further with the model chelate EDTA and the soil fungicide propicanazole. Third, a combined foliar application of SNP and gibberellic acid (GA3) was examined with EDTA and propicanazole. The soil application of propiconazole (a broad-spectrum fungicides) reduced AMF colonization and allowed greater Pb phytoextraction. The foliar application of SNP resulted in similar concentrations of Pb (roots and foliage) to plants that were challenged with chelates and soil fungicides. The combined foliar application of SNP and GA3 resulted in significantly greater average Pb concentration (243 mg kg−1) in plant foliage in comparison to control plants (182 mg kg−1) and plants treated with GA3 alone (202 mg kg−1). The combined foliar application of SNP and GA3 resulted in the greatest phytoextraction efficiency and could therefore potentially improve phytoextraction by switchgrass grown in Pb contaminated soils. 
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  5. The IceCube Neutrino Observatory at the South Pole has measured the diffuse astrophysical neutrino flux up to ~PeV energies and is starting to identify first point source candidates. The next generation facility, IceCube-Gen2, aims at extending the accessible energy range to EeV in order to measure the continuation of the astrophysical spectrum, to identify neutrino sources, and to search for a cosmogenic neutrino flux. As part of IceCube-Gen2, a radio array is foreseen that is sensitive to detect Askaryan emission of neutrinos beyond ~30 PeV. Surface and deep antenna stations have different benefits in terms of effective area, resolution, and the capability to reject backgrounds from cosmic-ray air showers and may be combined to reach the best sensitivity. The optimal detector configuration is still to be identified. This contribution presents the full-array simulation efforts for a combination of deep and surface antennas, and compares different design options with respect to their sensitivity to fulfill the science goals of IceCube-Gen2. 
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  6. The IceCube Neutrino Observatory opened the window on high-energy neutrino astronomy by confirming the existence of PeV astrophysical neutrinos and identifying the first compelling astrophysical neutrino source in the blazar TXS0506+056. Planning is underway to build an enlarged detector, IceCube-Gen2, which will extend measurements to higher energies, increase the rate of observed cosmic neutrinos and provide improved prospects for detecting fainter sources. IceCube-Gen2 is planned to have an extended in-ice optical array, a radio array at shallower depths for detecting ultra-high-energy (>100 PeV) neutrinos, and a surface component studying cosmic rays. In this contribution, we will discuss the simulation of the in-ice optical component of the baseline design of the IceCube-Gen2 detector, which foresees the deployment of an additional ~120 new detection strings to the existing 86 in IceCube over ~7 Antarctic summer seasons. Motivated by the phased construction plan for IceCube-Gen2, we discuss how the reconstruction capabilities and sensitivities of the instrument are expected to progress throughout its deployment. 
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